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Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions

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Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions1 Scope This International Standard defines terms for the detection of food borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This International Standard also specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real time PCR. The minimum requirements laid down in this International Standard provide the basis for

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