Protoplast Regeneration Medium

The addition of the MES buffer is to ensure that acidification during cultivation was prevented

fungi and higher eukaryotes by inhibiting protein synthesis

Synthetic Auxin

Solutions of Ascorbic Acid are light-sensitive and will quickly oxidize

Pyridoxine HCl: 1

serum and cell culture supernatants

X-Gal 5-Bromo-4-Chloro-3-Indolyl-ß-D-Galactopyranoside)

L-Arginine Hydrochloride acts as a precursor of nitric oxide synthesis that induces vasodilation in vivo

Contains 101g vitamin mix in prepared 1000 mL

or a convenient 1% sterile solution for use in plant tissue culture

• Transfer 1 ml of the above cell lysate

EGTA chelates these two ions at a 1:1 ratio